{Reference Type}: Journal Article
{Title}: Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface.
{Author}: Van Berkel GJ;Kertesz V;Boeltz H;
{Journal}: Bioanalysis
{Volume}: 9
{Issue}: 21
{Year}: Nov 2017
{Factor}: 2.695
{DOI}: 10.4155/bio-2017-0104
{Abstract}: <B>OBJECTIVE: </B>The aim of this work was to demonstrate and evaluate the analytical performance of coupling the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. Methodology & results: A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c.<BR><B>CONCLUSIONS: </B>This immediate drop on demand technology/open port sampling interface/ESI-MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.