{Reference Type}: Journal Article
{Title}: Characterization of the B Cell Transcriptome Bound by RNA-Binding Proteins with iCLIP.
{Author}: Díaz-Muñoz MD;Monzón-Casanova E;Turner M;
{Journal}: Methods Mol Biol
{Volume}: 1623
{Issue}: 0
{Year}: 2017
暂无{DOI}: 10.1007/978-1-4939-7095-7_14
{Abstract}: Posttranscriptional regulation of gene expression shapes the B cell transcriptome and controls messenger RNA (mRNA) translation into protein. Recent reports have highlighted the importance of RNA binding proteins (RBPs) for mRNA splicing, subcellular location, stability, and translation during B lymphocyte development, activation, and differentiation. Here we describe individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) in primary lymphocytes, a method that maps RNA-protein interactions in a genome-wide scale allowing mechanistic analysis of RBP function. We discuss the latest improvements in iCLIP technology and provide some examples of how integration of the RNA-protein interactome with other high-throughput mRNA sequencing methodologies uncovers the important role of RBP-mediated RNA regulation in key biological cell processes.