{Reference Type}: Journal Article {Title}: Generating Recombinant Pseudorabies Virus for Use as a Vaccine Platform. {Author}: Tan F;Li X;Tian K; {Journal}: Methods Mol Biol {Volume}: 1581 {Issue}: 0 {Year}: 2017 暂无{DOI}: 10.1007/978-1-4939-6869-5_5 {Abstract}: Pseudorabies virus (PRV) is a promising vaccine vector due to its distinctive features including many nonessential replication regions and a broad host range. Foreign genes of other viruses have been successfully inserted into and expressed in PRV and these recombinant viruses are very likely to induce humoral and/or cellular responses in immunized animals. This chapter offers an overview of methods for generating recombinant pseudorabies virus for use as a vaccine vector.