{Reference Type}: Journal Article
{Title}: [Exploratory study on influence of biopsy to biological behavior of Tca8113 transplanted tumor].
{Author}: Jiyuan L;Jian P;Chengge H;Yunlong W;Zhengwen L;Xiufa T;
{Journal}: Hua Xi Kou Qiang Yi Xue Za Zhi
{Volume}: 34
{Issue}: 6
{Year}: Dec 2016 1
暂无{DOI}: 10.7518/hxkq.2016.06.015
{Abstract}: OBJECTIVE: We established an animal model of nude mice with Tca8113 tumor and cut some tissue for biopsy. We also determined the biological behavior and mechanisms of the tumor.
METHODS: The mice were divided into two groups randomly. Mice in both groups were injected with Tca8113 cells into their tongues. The survival condition, growth of primary focus, and metastasis were observed. Hematoxylin and eosin staining and immunohistochemistry were performed on nuclear factor κB (NF-κB), matrix metallopeptidase 9 (MMP-9), vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), and Ki67 to determine their distributions within the tumor. Cytokeratin staining was also performed to detect micrometastasis in the submandibular lymph nodes.
RESULTS: The emerging rate of tumor was 97.92%. The weight and survival time of the experimental group were lower than that of the control group, whereas the metastasis ratio was higher. The expression of NF-κB, MMP-9, SDF-1, and MMP-9 in tumors was higher in the experimental group than that in the control group. The expression of NF-κB, MMP-9, VEGF, and SDF-1 was relevant. The microvessel density of the experimental group was higher than that in the control group.
CONCLUSIONS: Biopsy can affect the biological behavior of tongue tumor and can promote growth of primary focus and metastasis.
目的 通过建立裸鼠Tca8113移植瘤切取活检动物模型来了解活检对肿瘤生物学行为的影响,并初步探讨其机制。 方法 将裸鼠分为两组。舌部注射Tca8113细胞,成瘤后建立切取活检模型。观测裸鼠生存情况,原发灶生长及淋巴结转移状况,并通过免疫组织化学染色检测肿瘤原发灶核因子κB(NF-κB)、基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)、基质衍生因子-1(SDF-1)、细胞增殖抗原标记物(Ki67)表达水平及肿瘤淋巴结微转移情况。 结果 移植瘤成瘤率达97.92%。切取活检后实验组原发灶生长速度,淋巴结转移率,微血管密度,NF-κB、MMP-9及SDF-1表达均高于对照组。各项指标表达具有一定相关性。 结论 切取活检对肿瘤的生物学行为具有一定影响,可能加速原发灶的生长及转移。.