{Reference Type}: Journal Article
{Title}: Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.
{Author}: Marvin RK;Saepoo MB;Ye S;White DB;Liu R;Hensley K;Rega P;Kazan V;Giovannucci DR;Isailovic D;
{Journal}: Biomarkers
{Volume}: 22
{Issue}: 3
{Year}: May 2017 0
{Factor}: 2.663
{DOI}: 10.1080/1354750X.2017.1279215
{Abstract}: <B>BACKGROUND: </B>Quantitative changes of salivary proteins due to acute stress were detected.<BR><B>OBJECTIVE: </B>To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.<BR><B>METHODS: </B>Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.<BR><B>RESULTS: </B>Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.<BR><B>CONCLUSIONS: </B>Alpha-amylase and cystatins are potential salivary markers of acute stress, but further validation should be performed using larger sample populations.