{Reference Type}: Journal Article
{Title}: Interferometric Scattering Microscopy for the Study of Molecular Motors.
{Author}: Andrecka J;Takagi Y;Mickolajczyk KJ;Lippert LG;Sellers JR;Hancock WO;Goldman YE;Kukura P;
{Journal}: Methods Enzymol
{Volume}: 581
{Issue}: 0
{Year}: 2016
{Factor}: 1.682
{DOI}: 10.1016/bs.mie.2016.08.016
{Abstract}: Our understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20nm diameter. Using myosin-5, kinesin-1, and dynein as examples, we describe the basic assays, labeling strategies, and principles of data analysis. Our approach is relevant not only for motor protein dynamics but also provides a general tool for single-particle tracking with high spatiotemporal precision, which overcomes the limitations of single-molecule fluorescence methods.