{Reference Type}: Journal Article
{Title}: MicroRNA-2400 promotes bovine preadipocyte proliferation.
{Author}: Wei Y;Cui YF;Tong HL;Zhang WW;Yan YQ;
{Journal}: Biochem Biophys Res Commun
{Volume}: 478
{Issue}: 3
{Year}: 09 2016 23
{Factor}: 3.322
{DOI}: 10.1016/j.bbrc.2016.08.038
{Abstract}: MicroRNAs (miRNAs) play critical roles in the proliferation of bovine preadipocytes. miR-2400 is a novel and unique miRNA from bovines. In the present study, we separated and identified preadipocytes from bovine samples. miR-2400 overexpression increased the rate of preadipocyte proliferation, which was analyzed with a combination of EdU and flow cytometry. Simultaneously, functional genes related to proliferation (PCNA, CCND2, CCNB1) were also increased, which was detected by real-time PCR. Furthermore, luciferase reporter assays showed that miR-2400 bound directly to the 3'untranslated regions (3'UTRs) of PRDM11 mRNA. These data suggested that miR-2400 could promote preadipocyte proliferation by targeting PRDM11.