{Reference Type}: Journal Article
{Title}: Molecular analysis for bacterial contamination in dental unit water lines.
{Author}: Watanabe A;Tamaki N;Matsuyama M;Kokeguchi S;
{Journal}: New Microbiol
{Volume}: 39
{Issue}: 2
{Year}: Apr 2016
{Factor}: 1.383
{DOI}: 
{Abstract}: Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.