{Reference Type}: Journal Article
{Title}: Effect of amygdalin on proliferation of rat hepatic fat-storing cells and collagen production in vitro.
{Author}: Xu LM;Liu C;Liu P;
{Journal}: World J Gastroenterol
{Volume}: 3
{Issue}: 2
{Year}: Jun 1997 15
{Factor}: 5.374
{DOI}: 10.3748/wjg.v3.i2.103
{Abstract}: <B>OBJECTIVE: </B>To study the anti-fibrotic mechanism of amygdalin, a component of Semen Persicae, on fat-storing cells (FSC).<BR><B>METHODS: </B>Livers of normal adult rats were perfused with Pronas E and collagenase in situ. FSC were isolated by centrifugation with 11% Metrizamide. The subcultured FSC were incubated with 10(-4)-10(-8) mol/L amygdalin for 72 h, and then FSC proliferation and collagen production were assayed, respectively.<BR><B>RESULTS: </B>Low doses of amygdalin reduced incorporation of L-[(3)H]-thymidine into FSC and L-[5-(3)H]-proline into secreted collagenase-sensitive proteins and cell layer-associated collagenase-sensitive proteins. the strongest effects were seen for the 10(-8) mol/L dose of amygdalin, which inhibited the proliferation of FSC by 25.0%, and decreased collagen production in medium and cell layer by 24.2% and 26.8%, respectively.<BR><B>CONCLUSIONS: </B>An anti-fibrotic mechanism of amygdalin is to inhibit the proliferation and collagen production of active FSC.