{Reference Type}: Journal Article
{Title}: Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.
{Author}: Vogel M;Suess B;
{Journal}: Methods Mol Biol
{Volume}: 1380
{Issue}: 0
{Year}: 2016
暂无{DOI}: 10.1007/978-1-4939-3197-2_9
{Abstract}: Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.