{Reference Type}: Case Reports
{Title}: First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands.
{Author}: Bastian S;Nordmann P;Creton E;Malpote E;Thiery G;Martino F;Breurec S;Dortet L;
{Journal}: Int J Infect Dis
{Volume}: 34
{Issue}: 0
{Year}: May 2015
{Factor}: 12.074
{DOI}: 10.1016/j.ijid.2015.03.002
{Abstract}: <B>OBJECTIVE: </B>Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from Cuba<BR><B>METHODS: </B>Antibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assays<BR><B>RESULTS: </B>Here we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This K. pneumoniae isolate belongs to a new sequence type (ST1649). The blaNDM-1 gene together with the aacA4 gene were carried on a self conjugative IncR plasmid of c.a. 80kb.<BR><B>CONCLUSIONS: </B>This study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the blaNDM-1 carrying plasmid and the uncommon ST type of the K. pneumoniae strain suggest a possible local emergence of NDM producers.