{Reference Type}: Case Reports
{Title}: Characteristics of the mosaic genome of a human parechovirus type 1 strain isolated from an infant with pneumonia in China.
{Author}: Zhu R;Luo L;Zhao L;Deng J;Wang F;Sun Y;Song Q;Ding Y;Qian Y;
{Journal}: Infect Genet Evol
{Volume}: 29
{Issue}: 0
{Year}: Jan 2015
{Factor}: 4.393
{DOI}: 10.1016/j.meegid.2014.11.006
{Abstract}: Human parechoviruses (HPeVs) belong to the Parechovirus genus of the large and growing family of Picornaviridae with a non-enveloped, single-stranded and positive-sense RNA. An HPeV strain was isolated from the nasopharyngeal aspirate specimen of a 2 months old infant hospitalized with pneumonia in Beijing, China and nominated as BJ-37359 followed the code of the specimen. Strain BJ-37359 was identified as HPeV1 by whole genome sequencing. The full genome of strain BJ-37359 consisted of 7336 nucleotides (nt), excluding a poly (A) tail and contained an ORF of 6537 nt flanked by 5'UTR of 709 nt and 3'UTR of 90 nt. Phylogenetic analyses revealed that strain BJ-37359 were clustered together with HPeV1 strains in the structural capsid protein region, while uncoupling in the non-structural gene regions. Analyses with Simplot and Bootscan indicated that multiple recombination events occurred in the non-structural region and VP0 region of strain BJ-37359 with other HPeV1, and other types might have contributed to the recombination, especially HPeV6 and HPeV7 strains. Recombination analyses indicated that strain BJ-37359 may have a mosaic genome with new genomic recombination breakpoints.