{Reference Type}: Journal Article
{Title}: Immunoproteomic Analysis of the Excretory-Secretory Proteins from Spirometra mansoni Sparganum.
{Author}: Hu DD;Cui J;Wang L;Liu LN;Wei T;Wang ZQ;
{Journal}: Iran J Parasitol
{Volume}: 8
{Issue}: 3
{Year}: Jul 2013
{Factor}: 1.217
{DOI}: 
{Abstract}: <B>BACKGROUND: </B>Sparganosis is caused by the invasion of Spirometra sparganum into various tissues/organs. Subcutaneous sparganosis can be diagnosed by biopsy, while visceral/cerebral sparganosis is not easy to be diagnosed. The diagnosis depends largely on the detection of specific anti-sparganum antibodies. The specificity of the ELISA could be increased by using S. mansoni sparganum excretory-secretory (ES) antigens, but it also had the cross-reactions with sera of patients with cysticercosis or paragonimiasis. The aim of this study was to identify early specific diagnostic antigens in S. mansoni sparganum ES proteins.<BR><B>METHODS: </B>The sparganum ES proteins were analyzed by two-dimensional electrophoresis (2-DE) and Western blot probed with early sera from infected mice at 14 days post-infection. The immunoreactive protein spots were characterized by MALDI-TOF/ TOF-MS.<BR><B>RESULTS: </B>A total of approximately 149 proteins spots were detected with isoelectric point (pI) varying from 3 to 7.5 and molecular weight from 20 to 115 kDa and seven protein spots with molecular weight of 23-31 kDa were recognized by the infection sera. Three of seven spots were successfully identified and characterized as the same S. mansoni protein (cysteine protease), and the proteins of other 4 spots were not included in the databases.<BR><B>CONCLUSIONS: </B>The cysteine protease from S. mansoni ES proteins recognized by early infection sera might be the early diagnostic antigens for sparganosis.