{Reference Type}: Journal Article
{Title}: The role of Bowman's layer in corneal regeneration after phototherapeutic keratectomy: a prospective study using in vivo confocal microscopy.
{Author}: Lagali N;Germundsson J;Fagerholm P;
{Journal}: Invest Ophthalmol Vis Sci
{Volume}: 50
{Issue}: 9
{Year}: Sep 2009
{Factor}: 4.925
{DOI}: 10.1167/iovs.09-3781
{Abstract}: <B>OBJECTIVE: </B>To examine the role of Bowman's layer (BL) on the nature of anterior corneal regeneration after excimer laser phototherapeutic keratectomy (PTK).<BR><B>METHODS: </B>A cohort of 13 patients underwent PTK to remove either 7 mum of BL for treatment of primary recurrent corneal erosions (RCE; six patients) or complete BL removal (15-mum ablation) to treat RCE or poor vision secondary to map-dot-fingerprint (MDF) dystrophy (seven patients). Clinical examinations and laser-scanning in vivo confocal microscopy (IVCM) were conducted before surgery and at a mean of 4 and 8 months after surgery.<BR><B>RESULTS: </B>Total BL removal resulted in a significant decline in subbasal nerve density at 4 months (P = 0.007) that barely recovered to preoperative levels at 8 months (P = 0.055). With BL partially present, subbasal nerve density did not significantly change from preoperative levels. Superficial, wing, and basal epithelial cell density recovered to preoperative levels within 4 months after PTK, regardless of the presence of BL. Subepithelial keratocytes, however, were more densely distributed in corneas without BL relative to those with a partial BL present (P = 0.005), and increased anterior keratocyte reflectivity was noted in all eyes without BL and in no eye with a partial BL present.<BR><B>CONCLUSIONS: </B>Subbasal nerve regeneration is delayed and subepithelial keratocyte density and reflectivity remain elevated up to 10 months after total BL removal by PTK. The results provide initial evidence for a possible role of BL in facilitating rapid stromal wound healing and an associated recovery of anterior corneal transparency and the restoration of epithelial innervation after epithelial trauma.