{Reference Type}: Journal Article
{Title}: A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans.
{Author}: Lacourse EJ;Perally S;Hernandez-Viadel M;Wright HA;Brophy PM;
{Journal}: J Proteome Res
{Volume}: 7
{Issue}: 8
{Year}: Aug 2008
{Factor}: 5.37
{DOI}: 10.1021/pr8001035
{Abstract}: Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.