{Reference Type}: English Abstract
{Title}: [Determination of six major human herpes viruses in cerebrospinal fluid and blood of children with consensus primers].
{Author}: Dong GP;Shang SQ;Yu ZS;Liang L;Yu XL;
{Journal}: Zhejiang Da Xue Xue Bao Yi Xue Ban
{Volume}: 34
{Issue}: 1
{Year}: Jan 2005
暂无{Abstract}: <B>OBJECTIVE: </B>To identify 6 major human herpesviruses with consensus primers and to explore its clinical application.<BR><B>METHODS: </B>Based on the highly-homogeneous regions of DNA polymerase gene in human herpesviruses,Two pairs of primer were synthesized. One pair was designed to amplify herpes simplex virus type 1, type 2, Epstein-Barr virus and cytomegalovirus; and another was used to amplify varicella-zoster virus or human herpesvirus 6. Virus species identification was performed by restriction enzyme digestion with BamH I and BstU I. Thirty-eight CSF specimens of clinically diagnosed viral encephalitis,and 49 blood specimens from 27 confirmed cases and 22 clinically diagnosed ones were tested for herpes virus DNA using the PCR-RFLP assay with these primers.<BR><B>RESULTS: </B>Thirteen out of 38 CSF specimens (34.2%) were herpes virus positive. All blood specimens from 27 confirmed cases showed positive results, while for 22 clinically diagnosed cases 16 (72.7%) were positive. The types of herpes virus were determined using restriction enzyme digestion with BamH I and BstU I. Two CSF specimens from the patients, who were treated with aciclovir for 2 - 3 days, were still positive for herpes virus DNA by this method. None of the control blood or CSF controls were positive for herpesvirus by PCR.<BR><B>CONCLUSIONS: </B>The PCR-RFLP method used in this study is a specific, sensitive and practicable one for diagnosis of herpes virus infection.