{Reference Type}: Journal Article
{Title}: Synthesis of dolichyl phosphate derivatives with fluorescent label at the omega-end of the chain, new tools to study protein glycosylation.
{Author}: Shibaev VN;Veselovsky VV;Lozanova AV;Maltsev SD;Danilov LL;Forsee WT;Xing J;Cheung HC;Jedrzejas MJ;
{Journal}: Bioorg Med Chem Lett
{Volume}: 10
{Issue}: 2
{Year}: Jan 2000 17
{Factor}: 2.94
{DOI}: 10.1016/s0960-894x(99)00662-9
{Abstract}: Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the omega-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.