%0 Journal Article
%T Protocol for electrophysiological measurements of circadian changes in excitability in dentate granule cells from adult mice.
%A Gonzalez JC
%A Lee H
%A Overstreet-Wadiche L
%J STAR Protoc
%V 5
%N 3
%D 2024 Aug 14
%M 39146190
暂无%R 10.1016/j.xpro.2024.103255
%X Many types of neurons exhibit a daily rhythm of intrinsic excitability. Here, we present a protocol for assessing circadian regulation of dentate granule cell excitability using a mouse model for conditional knockout of the molecular clock protein BMAL1. We describe steps for obtaining healthy oblique horizontal slices that contain the hippocampus and measuring intrinsic excitability and synaptic potentials by combining whole-cell patch-clamp recordings and perforant-path electric stimulation. We then detail procedures for validating single-cell genetic deletion of Bmal1 by immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Gonzalez et al.1.