%0 Journal Article
%T Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.
%A Casabianca A
%A Bellina M
%A Dudgeon C
%A Nezhad MS
%A Harris C
%A Fiore S
%A Bernet A
%A Narrow W
%A Prela O
%A Wang L
%A Astsaturov I
%A Mehlen P
%A Carpizo DR
%J STAR Protoc
%V 5
%N 3
%D 2024 Aug 13
%M 39141519
暂无%R 10.1016/j.xpro.2024.103256
%X Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.1.