%0 Journal Article
%T High expression of oleoyl-ACP hydrolase underpins life-threatening respiratory viral diseases.
%A Jia X
%A Crawford JC
%A Gebregzabher D
%A Monson EA
%A Mettelman RC
%A Wan Y
%A Ren Y
%A Chou J
%A Novak T
%A McQuilten HA
%A Clarke M
%A Bachem A
%A Foo IJ
%A Fritzlar S
%A Carrera Montoya J
%A Trenerry AM
%A Nie S
%A Leeming MG
%A Nguyen THO
%A Kedzierski L
%A Littler DR
%A Kueh A
%A Cardamone T
%A Wong CY
%A Hensen L
%A Cabug A
%A Laguna JG
%A Agrawal M
%A Flerlage T
%A Boyd DF
%A Van de Velde LA
%A Habel JR
%A Loh L
%A Koay HF
%A van de Sandt CE
%A Konstantinov IE
%A Berzins SP
%A Flanagan KL
%A Wakim LM
%A Herold MJ
%A Green AM
%A Smallwood HS
%A Rossjohn J
%A Thwaites RS
%A Chiu C
%A Scott NE
%A Mackenzie JM
%A Bedoui S
%A Reading PC
%A Londrigan SL
%A Helbig KJ
%A Randolph AG
%A Thomas PG
%A Xu J
%A Wang Z
%A Chua BY
%A Kedzierska K
%J Cell
%V 187
%N 17
%D 2024 Aug 22
%M 39137778
%F 66.85
%R 10.1016/j.cell.2024.07.026
%X Respiratory infections cause significant morbidity and mortality, yet it is unclear why some individuals succumb to severe disease. In patients hospitalized with avian A(H7N9) influenza, we investigated early drivers underpinning fatal disease. Transcriptomics strongly linked oleoyl-acyl-carrier-protein (ACP) hydrolase (OLAH), an enzyme mediating fatty acid production, with fatal A(H7N9) early after hospital admission, persisting until death. Recovered patients had low OLAH expression throughout hospitalization. High OLAH levels were also detected in patients hospitalized with life-threatening seasonal influenza, COVID-19, respiratory syncytial virus (RSV), and multisystem inflammatory syndrome in children (MIS-C) but not during mild disease. In olah-/- mice, lethal influenza infection led to survival and mild disease as well as reduced lung viral loads, tissue damage, infection-driven pulmonary cell infiltration, and inflammation. This was underpinned by differential lipid droplet dynamics as well as reduced viral replication and virus-induced inflammation in macrophages. Supplementation of oleic acid, the main product of OLAH, increased influenza replication in macrophages and their inflammatory potential. Our findings define how the expression of OLAH drives life-threatening viral disease.