%0 Journal Article
%T Morphological correlates of synaptic protein turnover in the mouse brain.
%A Li F
%A Bahr JN
%A Bierth FA
%A Reshetniak S
%A Tetzlaff C
%A Fornasiero EF
%A Wichmann C
%A Rizzoli SO
%J Life Sci Alliance
%V 7
%N 11
%D 2024 11
%M 39134363
%F 5.781
%R 10.26508/lsa.202402793
%X Synaptic proteins need to be replaced regularly, to maintain function and to prevent damage. It is unclear whether this process, known as protein turnover, relates to synaptic morphology. To test this, we relied on nanoscale secondary ion mass spectrometry, to detect newly synthesized synaptic components in the brains of young adult (6 mo old) and aged mice (24 mo old), and on transmission electron microscopy, to reveal synapse morphology. Several parameters correlated to turnover, including pre- and postsynaptic size, the number of synaptic vesicles and the presence of a postsynaptic nascent zone. In aged mice, the turnover of all brain compartments was reduced by ∼20%. The turnover rates of the pre- and postsynapses correlated well in aged mice, suggesting that they are subject to common regulatory mechanisms. This correlation was poorer in young adult mice, in line with their higher synaptic dynamics. We conclude that synapse turnover is reflected by synaptic morphology.