%0 Journal Article
%T Towards a thorough understanding of mammalian glycosylphosphatidylinositol-anchored protein biosynthesis.
%A Kinoshita T
%J Glycobiology
%V 0
%N 0
%D 2024 Aug 12
%M 39129667
%F 5.954
%R 10.1093/glycob/cwae061
%X Glycosylphosphatidylinositols (GPIs) are glycolipids found ubiquitously in eukaryotes. They consist of a glycan and an inositol phospholipid, and act as membrane anchors of many cell-surface proteins by covalently linking to their C-termini. GPIs also exist as unlinked, free glycolipids on the cell surface. In human cells, at least 160 proteins with various functions are GPI-anchored proteins (GPI-APs). Because the attachment of GPI is required for the cell-surface expression of GPI-APs, a thorough knowledge of the molecular basis of mammalian GPI-AP biosynthesis is important for understanding the basic biochemistry and biology of GPI-APs and their medical significance. In this paper, I review our previous knowledge of the biosynthesis of mammalian GPI-APs and then examine new findings made since 2020.