%0 Journal Article
%T Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.
%A Padmarajan J
%A Edilyam AK
%A Subramanian VV
%J Methods Mol Biol
%V 2818
%N 0
%D 2024
%M 39126477
暂无%R 10.1007/978-1-0716-3906-1_14
%X Conditional depletion of proteins is a potential strategy to elucidate protein function, especially in complex cellular processes like meiosis. Several methods are available to effectively deplete a protein in a conditional manner. Conditional loss of a protein function can be achieved by depleting it from its region of action by degrading it. A conditional loss of protein function can also be achieved by sequestering it to a functionally unavailable compartment inside the cell. This chapter describes anchor away, a conditional depletion tool that can deplete a protein both temporally and spatially by translocation. It utilizes the affinity of FRB to bind FKBP12 in the presence of rapamycin for a quick and efficient translocation of the protein to a designated location. Anchor away is a reliable tool for the study of meiotic proteins, as only small quantities of rapamycin are required to efficiently and rapidly translocate the protein of interest without compromising meiotic progression.