%0 Journal Article
%T Screening of novel disease genes of sepsis-induced myocardial Disfunction by RNA sequencing and bioinformatics analysis.
%A Yao H
%A Xiao Z
%A Liu S
%A Gao X
%A Wu Z
%A Li D
%A Yi Z
%A Zhou H
%A Zhang W
%J Genomics
%V 116
%N 5
%D 2024 Aug 5
%M 39111545
%F 4.31
%R 10.1016/j.ygeno.2024.110911
%X <B>BACKGROUND: </B>There is still a lack of effective treatment for sepsis-induced myocardial dysfunction (SIMD), while the pathogenesis of SIMD still remains largely unexplained.<BR><B>METHODS: </B>RNA sequencing results (GSE267388 and GSE79962) were used for cross-species integrative analysis. Bioinformatic analyses were used to delve into function, tissue- and cell- specificity, and interactions of genes. External datasets and qRT-PCR experiments were used for validation. L1000 FWD was used to predict targeted drugs, and 3D structure files were used for molecular docking.<BR><B>RESULTS: </B>Based on bioinformatic analyses, ten differentially expressed genes were selected as genes of interest, seven of which were verified to be significantly differential expression. Bucladesine was considered as a potential targeted drug for SIMD, which banded to seven target proteins primarily by forming hydrogen bonds.<BR><B>CONCLUSIONS: </B>It was considered that Cebpd, Timp1, Pnp, Osmr, Tgm2, Cp, and Asb2 were novel disease genes, while bucladesine was a potential therapeutic drug, of SIMD.