%0 Journal Article
%T Regulation of MYC by CARD14 in human epithelium is a determinant of epidermal homeostasis and disease.
%A DeVore SB
%A Schuetz M
%A Alvey L
%A Lujan H
%A Ochayon DE
%A Williams L
%A Chang WC
%A Filuta A
%A Ruff B
%A Kothari A
%A Hahn JM
%A Brandt E
%A Satish L
%A Roskin K
%A Herr AB
%A Biagini JM
%A Martin LJ
%A Cagdas D
%A Keles S
%A Milner JD
%A Supp DM
%A Khurana Hershey GK
%J Cell Rep
%V 43
%N 8
%D 2024 Aug 27
%M 39110589
暂无%R 10.1016/j.celrep.2024.114589
%X Caspase recruitment domain family member 14 (CARD14) and its variants are associated with both atopic dermatitis (AD) and psoriasis, but their mechanistic impact on skin barrier homeostasis is largely unknown. CARD14 is known to signal via NF-κB; however, CARD14-NF-κB signaling does not fully explain the heterogeneity of CARD14-driven disease. Here, we describe a direct interaction between CARD14 and MYC and show that CARD14 signals through MYC in keratinocytes to coordinate skin barrier homeostasis. CARD14 directly binds MYC and influences barrier formation in an MYC-dependent fashion, and this mechanism is undermined by disease-associated CARD14 variants. These studies establish a paradigm that CARD14 activation regulates skin barrier function by two distinct mechanisms, including activating NF-κB to bolster the antimicrobial (chemical) barrier and stimulating MYC to bolster the physical barrier. Finally, we show that CARD14-dependent MYC signaling occurs in other epithelia, expanding the impact of our findings beyond the skin.