%0 Journal Article
%T Synergistic enhancement of fluorescein-K3[Fe(CN)6] CL by MoO3-x NPs for sensitive and noninvasive detection of uric acid in saliva.
%A Chen Y
%A Yang Z
%A Qi J
%A Chen F
%J Mikrochim Acta
%V 191
%N 9
%D 2024 08 7
%M 39110277
%F 6.408
%R 10.1007/s00604-024-06585-w
%X MoO3-x NPs was rapidly synthesized at room temperature by an easy stirring method. It was interesting to find that MoO3-x NPs induce OH- to generate active free radicals (ROS), which is a highly promising property in chemiluminescence (CL). Benefiting from the abundant oxygen vacancy, MoO3-x NPs adsorbs H2O2 and turn it into ·OH. The oxidase activity of fluorescein under visible light had already been reported, which catalyzes dissolved oxygen to become O2-· and continue to convert to H2O2. By creating the synergy effect with fluorescein, MoO3-x NPs strengthen the CL intensity of K3[Fe(CN)6]-fluorescein system significantly. Utilizing the quench effect of uric acid for the CL intensity, we developed a rapid, simple, and highly sensitive CL platform for uric acid detection. The linear range was 5-80 µM and the detection limit (LOD) for uric acid was 3.11 µM (S/N = 3). This work expanded the application of MoO3-x NPs in the CL field and developed a simple and highly sensitive CL sensing system to detect UA in human saliva.