%0 Journal Article %T Joint GWAS and WGCNA Identify Genes Regulating the Isoflavone Content in Soybean Seeds. %A Yang Z %A Wu X %A Zhu Y %A Qu Y %A Zhou C %A Yuan M %A Zhan Y %A Li Y %A Teng W %A Zhao X %A Han Y %J J Agric Food Chem %V 72 %N 33 %D 2024 Aug 21 %M 39105709 %F 5.895 %R 10.1021/acs.jafc.4c03012 %X Isoflavone is a secondary metabolite of the soybean phenylpropyl biosynthesis pathway with physiological activity and is beneficial to human health. In this study, the isoflavone content of 205 soybean germplasm resources from 3 locations in 2020 showed wide phenotypic variation. A joint genome-wide association study (GWAS) and weighted gene coexpression network analysis (WGCNA) identified 33 single-nucleotide polymorphisms and 11 key genes associated with soybean isoflavone content. Gene ontology enrichment analysis, gene coexpression, and haplotype analysis revealed natural variations in the Glyma.12G109800 (GmOMT7) gene and promoter region, with Hap1 being the elite haplotype. Transient overexpression and knockout of GmOMT7 increased and decreased the isoflavone content, respectively, in hairy roots. The combination of GWAS and WGCNA effectively revealed the genetic basis of soybean isoflavone and identified potential genes affecting isoflavone synthesis and accumulation in soybean, providing a valuable basis for the functional study of soybean isoflavone.