%0 Journal Article
%T Biocatalytic Method for Producing an Affinity Resin for the Isolation of Immunoglobulins.
%A Tereshin MN
%A Melikhova TD
%A Eletskaya BZ
%A Ivanova EA
%A Onoprienko LV
%A Makarov DA
%A Razumikhin MV
%A Myagkikh IV
%A Fabrichniy IP
%A Stepanenko VN
%J Biomolecules
%V 14
%N 7
%D 2024 Jul 14
%M 39062563
%F 6.064
%R 10.3390/biom14070849
%X Affinity chromatography is a widely used technique for antibody isolation. This article presents the successful synthesis of a novel affinity resin with a mutant form of protein A (BsrtA) immobilized on it as a ligand. The key aspect of the described process is the biocatalytic immobilization of the ligand onto the matrix using the sortase A enzyme. Moreover, we used a matrix with primary amino groups without modification, which greatly simplifies the synthesis process. The resulting resin shows a high dynamic binding capacity (up to 50 mg IgG per 1 mL of sorbent). It also demonstrates high tolerance to 0.1 M NaOH treatment and maintains its effectiveness even after 100 binding, elution, and sanitization cycles.