%0 Journal Article
%T An analysis of the vaginal microbiota and cervicovaginal metabolomics in cervical lesions and cervical carcinoma.
%A Ou J
%A Kang Y
%A Medlegeh
%A Fu K
%A Zhang Y
%A Yang W
%J Heliyon
%V 10
%N 13
%D 2024 Jul 15
%M 39040371
%F 3.776
%R 10.1016/j.heliyon.2024.e33383
%X UNASSIGNED: To explore the role of vaginal microbiota and metabolomics in the progression of cervical dysplasia.
UNASSIGNED: The patient group consists of female patients with low-grade, high-grade cervical dysplasia, and cervical cancer. Normal cervix samples from health volunteers were used as controls. The metabolic fingerprints of cervicovaginal lavage were analyzed using liquid chromatography-mass spectrometry, while the vaginal microbiota was examined through 16S rRNA sequencing. Bioinformatic analysis was adopted to investigate the interplay between hosts and microbes. The vaginal metabolic and microbiota profiles of 90 female patients with cervical dysplasia and 10 controls were analyzed to discover the biological characteristics underlying the progression of cervical cancer.
UNASSIGNED: We found that Valyl-Glutamate, N, N'-Diacetylbenzidine, and Oxidized glutathione, which were involved in oxidative stress response, were discriminators to distinguish the normal cervix, invasive cervical carcinomas, and CIN3 from others. Cervical carcinoma was characterized by a large variety of vaginal microbes (dominated by non-Lactobacillus communities) compared to the control. These microbes affected amino acid and nucleotide metabolism, producing metabolites with cervical carcinoma and genital inflammation compared to the control group.
UNASSIGNED: This study revealed that cervicovaginal metabolic profiles were determined by cervical cancer, vaginal microbiota, and their interplays. ROS metabolism can be used to discriminate normal cervix, CIN3, and invasive cervical carcinoma.