%0 Journal Article
%T The bioconversion of dietary α-linolenic acid to eicosapentaenoic acid in Bombyx mori.
%A Ohhara Y
%A Sato M
%A Sakai M
%A Sugiyama C
%A Ozawa T
%A Yamakawa-Kobayashi K
%J Comp Biochem Physiol B Biochem Mol Biol
%V 274
%N 0
%D 2024 Oct-Dec 20
%M 39038779
%F 2.495
%R 10.1016/j.cbpb.2024.111007
%X n-3 Long-chain polyunsaturated fatty acids (n-3 LC-PUFAs), including eicosapentaenoic acid (EPA), are essential multifunctional nutrients in animals. Microorganisms such as microalgae are known to be n-3 LC-PUFA producers in aquatic environments. Various aquatic invertebrates, including Harpacticoida copepods, and a few terrestrial invertebrates, such as the nematode Caenorhabditis elegans, possess n-3 LC-PUFA biosynthetic enzymes. However, the capacity for n-3 LC-PUFA biosynthesis and the underlying molecular mechanisms in terrestrial insects are largely unclear. In this study, we investigated the fatty acid biosynthetic pathway in the silkworm Bombyx mori and found that EPA was present in silkworms throughout their development. Stable isotope tracing revealed that dietary α-linolenic acid (ALA) was metabolized to EPA in silkworm larvae. These results indicated that silkworms synthesize EPA from ALA. Given that EPA is enriched in the central nervous system, we propose that EPA confers optimal neuronal functions, similar to docosahexaenoic acid, in the mammalian nervous system.