%0 Journal Article
%T Rapid, Multiplexed, and Enzyme-Free Nucleic Acid Detection Using Programmable Aptamer-Based RNA Switches.
%A Yan Z
%A Eshed A
%A Tang AA
%A Arevalos NR
%A Ticktin ZM
%A Chaudhary S
%A Ma D
%A McCutcheon G
%A Li Y
%A Wu K
%A Saha S
%A Alcantar-Fernandez J
%A Moreno-Camacho JL
%A Campos-Romero A
%A Collins JJ
%A Yin P
%A Green AA
%J Chem
%V 10
%N 7
%D 2024 Jul 11
%M 39036067
暂无%R 10.1016/j.chempr.2024.03.015
%X Rapid, simple, and low-cost diagnostic technologies are crucial tools for combatting infectious disease. We describe a class of aptamer-based RNA switches or aptaswitches that recognize target nucleic acid molecules and initiate folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide an intense fluorescent readout without intervening enzymes, generating signals in as little as 5 minutes and enabling detection by eye with minimal equipment. Aptaswitches can be used to regulate folding of seven fluorogenic aptamers, providing a general means of controlling aptamers and an array of multiplexable reporter colors. Coupling isothermal amplification reactions with aptaswitches, we reach sensitivities down to 1 RNA copy/μL in one-pot reactions. Application of multiplexed all-in-one reactions against RNA from clinical saliva samples yields an overall accuracy of 96.67% for detection of SARS-CoV-2 in 30 minutes. Aptaswitches are thus versatile tools for nucleic acid detection that are readily integrated into rapid diagnostic assays.