%0 Dataset %T Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae. %A Cao LJ %A Guan TB %A Chen JC %A Yang F %A Liu JX %A Jin FL %A Wei SJ %J Sci Data %V 11 %N 1 %D 2024 Jul 18 %M 39025916 %F 8.501 %R 10.1038/s41597-024-03640-2 %X The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a notorious pest in agriculture that has developed resistance to almost all chemical types used for its control. Here, we assembled a chromosome-level genome for the TSSM using Illumina, Nanopore, and Hi-C sequencing technologies. The assembled contigs had a total length of 103.94 Mb with an N50 of 3.46 Mb, with 87.7 Mb of 34 contigs anchored to three chromosomes. The chromosome-level genome assembly had a BUSCO completeness of 94.8%. We identified 15,604 protein-coding genes, with 11,435 genes that could be functionally annotated. The high-quality genome provides invaluable resources for the genetic and evolutionary study of TSSM.