%0 Journal Article
%T The activation of adenosine monophosphate-activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin-1 via epithelial-mesenchymal transition.
%A Zhou XY
%A Liu QM
%A Li Z
%A Liu XY
%A Zhao QW
%A Wang Y
%A Wu FH
%A Zhao G
%A Sun R
%A Guo XH
%J Animal Model Exp Med
%V 0
%N 0
%D 2024 Jul 17
%M 39017036
暂无%R 10.1002/ame2.12444
%X <B>BACKGROUND: </B>The role of Claudin-1 in tongue squamous cell carcinoma (TSCC) metastasis needs further clarification, particularly its impact on cell migration. Herein, our study aims to investigate the role of Claudin-1 in TSCC cell migration and its underlying mechanisms.<BR><B>METHODS: </B>36 TSCC tissue samples underwent immunohistochemical staining for Claudin-1. Western blotting and immunofluorescence analyses were conducted to evaluate Claudin-1 expression and distribution in TSCC cells. Claudin-1 knockdown cell lines were established using short hairpin RNA transfection. Migration effects were assessed through wound healing assays. Furthermore, the expression of EMT-associated molecules was measured via western blotting.<BR><B>RESULTS: </B>Claudin-1 expression decreased as TSCC malignancy increased. Adenosine monophosphate-activated protein kinase (AMPK) activation led to increased Claudin-1 expression and membrane translocation, inhibiting TSCC cell migration and epithelial-mesenchymal transition (EMT). Conversely, Claudin-1 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.<BR><B>CONCLUSIONS: </B>Our results indicated that AMPK activation suppresses TSCC cell migration by targeting Claudin-1 and EMT pathways.