%0 Journal Article
%T Rab10-CAV1 mediated intraluminal vesicle transport to migrasomes.
%A Li Y
%A Wen Y
%A Li Y
%A Tan X
%A Gao S
%A Fan P
%A Tian W
%A Wong CCL
%A Chen Y
%J Proc Natl Acad Sci U S A
%V 121
%N 30
%D 2024 Jul 23
%M 39008679
%F 12.779
%R 10.1073/pnas.2319267121
%X Migrasomes, vesicular organelles generated on the retraction fibers of migrating cells, play a crucial role in migracytosis, mediating intercellular communication. The cargoes determine the functional specificity of migrasomes. Migrasomes harbor numerous intraluminal vesicles, a pivotal component of their cargoes. The mechanism underlying the transportation of these intraluminal vesicles to the migrasomes remains enigmatic. In this study, we identified that Rab10 and Caveolin-1 (CAV1) mark the intraluminal vesicles in migrasomes. Transport of Rab10-CAV1 vesicles to migrasomes required the motor protein Myosin Va and adaptor proteins RILPL2. Notably, the phosphorylation of Rab10 by the kinase LRRK2 regulated this process. Moreover, CSF-1 can be transported to migrasomes through this mechanism, subsequently fostering monocyte-macrophage differentiation in skin wound healing, which served as a proof of the physiological importance of this transporting mechanism.