%0 Journal Article
%T Radiolabeling Heme and Porphyrin with 14C-Glycine or 14C δ-Aminolevulinic Acid.
%A Ward DM
%A Gillis S
%A Yien YY
%J Methods Mol Biol
%V 2839
%N 0
%D 2024
%M 39008256
暂无%R 10.1007/978-1-0716-4043-2_12
%X Radiolabeling enables the quantitation of newly synthesized heme and porphyrin, allowing us to distinguish heme synthesis rates from total cellular heme. Here, we describe a protocol for labeling heme with 14C-glycine or ALA and the sequential extraction of heme and porphyrin from the same samples for quantitation by liquid scintillation.