%0 Journal Article
%T The architecture of silk-secreting organs during the final larval stage of silkworms revealed by single-nucleus and spatial transcriptomics.
%A Ma Y
%A Li Q
%A Tang Y
%A Zhang Z
%A Liu R
%A Luo Q
%A Wang Y
%A Hu J
%A Chen Y
%A Li Z
%A Zhao C
%A Ran Y
%A Mu Y
%A Li Y
%A Xu X
%A Gong Y
%A He Z
%A Ba Y
%A Guo K
%A Dong K
%A Li X
%A Tan W
%A Zhu Y
%A Xiang Z
%A Xu H
%J Cell Rep
%V 43
%N 7
%D 2024 Jul 23
%M 38996068
暂无%R 10.1016/j.celrep.2024.114460
%X Natural silks are renewable proteins with impressive mechanical properties and biocompatibility that are useful in various fields. However, the cellular and spatial organization of silk-secreting organs remains unclear. Here, we combined single-nucleus and spatially resolved transcriptomics to systematically map the cellular and spatial composition of the silk glands (SGs) of mulberry silkworms late in larval development. This approach allowed us to profile SG cell types and cell state dynamics and identify regulatory networks and cell-cell communication related to efficient silk protein synthesis; key markers were validated via transgenic approaches. Notably, we demonstrated the indispensable role of the ecdysone receptor (ultraspiracle) in regulating endoreplication in SG cells. Our atlas presents the results of spatiotemporal analysis of silk-secreting organ architecture late in larval development; this atlas provides a valuable reference for elucidating the mechanism of efficient silk protein synthesis and developing sustainable products made from natural silk.