%0 Journal Article
%T Thrombocytopenia in SFTS due to platelets with altered function undergoing cell death pathways.
%A Fang Y
%A Shen S
%A Zhang J
%A Xu L
%A Wang T
%A Fan L
%A Zhu Q
%A Xiao J
%A Wu X
%A Jin J
%A Wu Q
%A Zhang Y
%A Tang S
%A Zheng X
%A Deng F
%J J Infect Dis
%V 0
%N 0
%D 2024 Jul 12
%M 38996045
%F 7.759
%R 10.1093/infdis/jiae355
%X BACKGROUND: Thrombocytopenia is the major clinical feature associated with the severity of SFTS, but the mechanism by which it occurs remains unclear.
METHODS: RNA transcriptome analyses were performed on platelets purified from SFTS patients and SFTSV-infected mice. The functions of differentially expressed genes (DEGs) in the platelets were characterized. ELISA, flow cytometry, and qRT-PCR were used to measure the levels of platelet activation, SFTSV infection in platelets, formation of neutrophil extracellular traps (NETs), transcription of DEGs and percent of platelets undergoing cell death.
RESULTS: Enhanced neutrophil activation and interferon (IFN) signaling involved in the viral life cycle were common platelet responses in SFTS, which may consume increasing numbers of platelets. Other functional changes may be associated with different outcomes of SFTS. SFTSV infection led to platelet destruction by pyroptosis, apoptosis, necroptosis, and autophagy. In contrast to SFTS patients, platelets in SFTSV-infected mice mainly play a role in adaptive immunity, and platelet death was not as severe as in humans.
CONCLUSIONS: The altered functions of platelets, such as mediating leukocyte activation and undergoing cell death, contribute to thrombocytopenia in SFTS patients. The different mechanisms of thrombocytopenia in mice, suggest that platelet functions should be considered in experimental animal models.