%0 Journal Article
%T High Probability of Lynch Syndrome Among Colorectal Cancer Patients Is Associated With Higher Occurrence of KRAS and PIK3CA Mutations.
%A Heriyanto DS
%A Yoshuantari N
%A Akbariani G
%A Lau V
%A Hanini H
%A Hidayati Z
%A Arief MZ
%A Gunawan AN
%A Ridwanuloh AM
%A Kusharyoto W
%A Handaya AY
%A Ilyas M
%A Kurnianda J
%A Hutajulu SH
%A Susanti S
%J World J Oncol
%V 15
%N 4
%D 2024 Aug
%M 38993255
暂无%R 10.14740/wjon1843
%X <B>UNASSIGNED: </B>In Indonesia, early-onset colorectal cancer (EOCRC) rates are higher in patients < 50 years old compared to Western populations, possibly due to a higher frequency of Lynch syndrome (LS) in CRC patients. We aimed to examine the association of KRAS and PIK3CA mutations with LS.<BR><B>UNASSIGNED: </B>In this retrospective cross-sectional single-center study, the PCR-HRM-based test was used for screening of microsatellite instability (MSI) mononucleotide markers (BAT25, BAT26, BCAT25, MYB, EWSR1), MLH1 promoter methylation, and oncogene mutations of BRAF (V600E), KRAS (exon 2 and 3), and PIK3CA (exon 9 and 20) in FFPE DNA samples.<BR><B>UNASSIGNED: </B>All the samples (n = 244) were from Dr. Sardjito General Hospital Yogyakarta, Indonesia. KRAS and PIK3CA mutations were found in 151/244 (61.88%) and 107/244 (43.85%) of samples, respectively. KRAS and PIK3CA mutations were significantly associated with MSI status in 32/42 (76.19%) and 25/42 (59.52%) of samples, respectively. KRAS mutation was significantly associated with LS status in 26/32 (81.25%) of samples. The PIK3CA mutation was present in a higher proportion in LS samples of 19/32 (59.38%), but not statistically significant. Clinicopathology showed that KRAS mutation was significantly associated with right-sided CRC and higher histology grade in 39/151 (25.83%) and 24/151 (16.44%) samples, respectively. PIK3CA mutation was significantly associated with female sex and lower levels of tumor-infiltrating lymphocytes in 62/107 (57.94%) and 26/107 (30.23%) samples, respectively. KRAS and PIK3CA mutations did not significantly affect overall survival (120 months) in LS and non-LS patients.<BR><B>UNASSIGNED: </B>The high probability of LS in Indonesian CRC patients is associated with KRAS and PIK3CA mutations.