%0 Journal Article
%T Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland.
%A Nguyen YDH
%A Pham TLA
%A Nishihara T
%A Kamei K
%A Tran DB
%J Arch Insect Biochem Physiol
%V 116
%N 3
%D 2024 Jul
%M 38993002
%F 2.454
%R 10.1002/arch.22132
%X Perilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of Lsd-1 in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd-1 genetically interacted with Myc in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.