%0 Journal Article
%T Using the High-Entropy Approach to Obtain Multimetal Oxide Nanozymes: Library Synthesis, In Silico Structure-Activity, and Immunoassay Performance.
%A Phan-Xuan T
%A Schweidler S
%A Hirte S
%A Schüller M
%A Lin L
%A Khandelwal A
%A Wang K
%A Schützke J
%A Reischl M
%A Kübel C
%A Hahn H
%A Bello G
%A Kirchmair J
%A Aghassi-Hagmann J
%A Brezesinski T
%A Breitung B
%A Dailey LA
%J ACS Nano
%V 18
%N 29
%D 2024 Jul 23
%M 38985736
%F 18.027
%R 10.1021/acsnano.4c03053
%X High-entropy nanomaterials exhibit exceptional mechanical, physical, and chemical properties, finding applications in many industries. Peroxidases are metalloenzymes that accelerate the decomposition of hydrogen peroxide. This study uses the high-entropy approach to generate multimetal oxide-based nanozymes with peroxidase-like activity and explores their application as sensors in ex vivo bioassays. A library of 81 materials was produced using a coprecipitation method for rapid synthesis of up to 100 variants in a single plate. The A and B sites of the magnetite structure, (AA')(BB'B'')2O4, were substituted with up to six different cations (Cu/Fe/Zn/Mg/Mn/Cr). Increasing the compositional complexity improved the catalytic performance; however, substitutions of single elements also caused drastic reductions in the peroxidase-like activity. A generalized linear model was developed describing the relationship between material composition and catalytic activity. Binary interactions between elements that acted synergistically or antagonistically were identified, and a single parameter, the mean interaction effect, was observed to correlate highly with catalytic activity, providing a valuable tool for the design of high-entropy-inspired nanozymes.