%0 Journal Article
%T Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation.
%A Hayashi T
%A Sadaki S
%A Tsuji R
%A Okada R
%A Fuseya S
%A Kanai M
%A Nakamura A
%A Okamura Y
%A Muratani M
%A Wenchao G
%A Sugasawa T
%A Mizuno S
%A Warabi E
%A Kudo T
%A Takahashi S
%A Fujita R
%J Stem Cells
%V 42
%N 9
%D 2024 Sep 10
%M 38975693
%F 5.845
%R 10.1093/stmcls/sxae045
%X Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, the direct target of MYOD in the process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that MyoD targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13:Dusp27 double knock-out mice, the ability for muscle regeneration after injury was reduced. Moreover, single-cell RNA sequencing of MyoD-high expressing MuSCs from MyoD-KI mice revealed that Dusp13 and Dusp27 are expressed only in specific populations within MyoD-high MuSCs, which also express Myogenin. Overexpressing Dusp13 in MuSCs causes premature muscle differentiation. Thus, we propose a model where DUSP13 and DUSP27 contribute to the fate transition of MuSCs from proliferation to differentiation during myogenesis.