%0 Journal Article
%T Development of Imiquimod-induced HaCaT-THP-1 co-culture for modeling of psoriasis.
%A Váradi J
%A Oláh B
%A Hosszú D
%A Fenyvesi F
%A Remenyik J
%A Homoki J
%A Nagy B
%A Fejes Z
%A Bácskay I
%A Klusóczki Á
%J Eur J Pharm Sci
%V 200
%N 0
%D 2024 Sep 1
%M 38972610
%F 5.112
%R 10.1016/j.ejps.2024.106846
%X Psoriasis is one of the most prevalent and chronic inflammatory disease of the skin, associated with disrupted barrier function. Currently, a widely accepted, generally usable cell culture model has not been developed yet. In the present work, we aimed to establish a co-culture model with human keratinocyte (HaCaT) and human monocyte cells (THP-1) induced by Imiquimod (IMQ), which acts on the TLR7 receptor. The role of TLR7 expressed on THP-1 cells was confirmed by immunofluorescence staining of NF-κB activation. Chloroquine (CH) was used as a receptor inhibitor, in the presence or absence of which the NF-κB pathway was activated. We determined the most effective proliferation-stimulating IMQ concentration by RTCA method and the hyperproliferative effect was investigated by wound-healing test. The effect of IMQ was compared with the effects of the anthocyanin (AC) components from the anti-inflammatory sour cherry extract that we have already studied. We found that IMQ significantly increased the migration rate however, the combined treatment resulted in a decreased migration rate compared to the IMQ treatment alone. Inflammatory cytokines were measured from the supernatant of co-culture by ELISA. During the development of the co-culture intended to model psoriasis, we confirmed the induction effect of IMQ and in the case of AC treatment, we supported the stabilizing effect of the barrier.