%0 Journal Article
%T The First Steps of the Visual Cycle in Human Rod and Cone Photoreceptors.
%A Chen C
%A Adler L
%A Milliken C
%A Rahman B
%A Kono M
%A Perry LP
%A Gonzalez-Fernandez F
%A Koutalos Y
%J Invest Ophthalmol Vis Sci
%V 65
%N 8
%D 2024 Jul 1
%M 38958967
%F 4.925
%R 10.1167/iovs.65.8.9
%X <B>UNASSIGNED: </B>Light detection destroys the visual pigment. Its regeneration, necessary for the recovery of light sensitivity, is accomplished through the visual cycle. Release of all-trans retinal by the light-activated visual pigment and its reduction to all-trans retinol comprise the first steps of the visual cycle. In this study, we determined the kinetics of all-trans retinol formation in human rod and cone photoreceptors.<BR><B>UNASSIGNED: </B>Single living rod and cone photoreceptors were isolated from the retinas of human cadaver eyes (ages 21 to 90 years). Formation of all-trans retinol was measured by imaging its outer segment fluorescence (excitation, 360 nm; emission, >420 nm). The extent of conversion of released all-trans retinal to all-trans retinol was determined by measuring the fluorescence excited by 340 and 380 nm. Measurements were repeated with photoreceptors isolated from Macaca fascicularis retinas. Experiments were carried out at 37°C.<BR><B>UNASSIGNED: </B>We found that ∼80% to 90% of all-trans retinal released by the light-activated pigment is converted to all-trans retinol, with a rate constant of 0.24 to 0.55 min-1 in human rods and ∼1.8 min-1 in human cones. In M. fascicularis rods and cones, the rate constants were 0.38 ± 0.08 min-1 and 4.0 ± 1.1 min-1, respectively. These kinetics are several times faster than those measured in other vertebrates. Interphotoreceptor retinoid-binding protein facilitated the removal of all-trans retinol from human rods.<BR><B>UNASSIGNED: </B>The first steps of the visual cycle in human photoreceptors are several times faster than in other vertebrates and in line with the rapid recovery of light sensitivity exhibited by the human visual system.