%0 Journal Article
%T Recombinant AAV Purification.
%A Joshi PRH
%A Venereo-Sanchez A
%J Methods Mol Biol
%V 2829
%N 0
%D 2024
%M 38951337
暂无%R 10.1007/978-1-0716-3961-0_15
%X Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as host cell proteins, host cell DNA, and other cell culture-related impurities. Here we describe the purification of rAAV produced in insect cells Sf9/rBEV by immuno-affinity capture chromatography. The method is fully scale-amenable unlike other traditional purification methods based on ultracentrifugation. The method reported herein has two main steps: (1) the clarification of cell lysate by depth filtration and (2) the selective capture and single-step purification of AAV via immune-affinity chromatography. This purification method has been successfully implemented to purify the majority of wild-type AAV serotypes.