%0 English Abstract
%T [Effects of polyphyllin Ⅶ on proliferation, apoptosis and cell cycle of diffuse large B-cell lymphoma cells].
%A Sun YQ
%A Fu Y
%A Ji OX
%A Wang LJ
%J Zhonghua Xue Ye Xue Za Zhi
%V 45
%N 4
%D 2024 Apr 14
%M 38951069
暂无%R 10.3760/cma.j.cn121090-20230831-00099
%X The aim of this study was to investigate the effects of polyphyllin Ⅶ (PP Ⅶ) on proliferation, apoptosis, and cell cycle of diffuse large B-cell lymphoma (PLBCL) cell lines U2932 and SUDHL-4. The DLBCL cell lines were divided into a control group and a PPⅦ group, and experiments were conducted using MTT assay, flow cytometry, and Western blotting.Results showed that compared with the control group, PPⅦ significantly inhibited the proliferation of U2932 and SUDHL-4 cells (P<0.05). Apoptosis assays demonstrated that treatment with 0.50 and 1.00 µmol/L PP Ⅶ significantly increased the apoptosis rates of both cell lines (P<0.05), upregulated apoptosis-related proteins, and downregulated Bcl-2 protein level (P<0.05). Cell cycle analysis revealed that PPⅦ treatment led to an increase in G0/G1-phase cells (P<0.05) and a decrease in G2/M-phase cells (P<0.05), significantly downregulated cyclin D1, CDK4, CDK6, and survivin protein expression (P<0.05). In conclusion, PPⅦ exerted anti-lymphoma effects by inhibiting proliferation, promoting apoptosis, and inducing G0/G1 phase arrest in DLBCL cells.<BR>本研究旨在探究重楼皂苷Ⅶ（PPⅦ）对弥漫大B细胞淋巴瘤（DLBCL）细胞株U2932和SUDHL-4的增殖、凋亡和细胞周期的影响。实验将DLBCL细胞株分为对照组和PPⅦ组，并使用MTT法、流式细胞术和Western blot法进行实验。结果显示，与对照组相比，PPⅦ显著抑制了U2932和SUDHL-4细胞的增殖（P<0.05）。细胞凋亡实验表明，0.5和1 µmol/L的PPⅦ处理使得两种细胞的凋亡率显著增加（P<0.05），并且凋亡相关蛋白的表达上调，而Bcl-2蛋白水平显著降低（P<0.05）。细胞周期实验显示，PPⅦ处理使得G0/G1期细胞增加（P<0.05），G2/M期细胞减少（P<0.05），且Cyclin D1、CDK4、CDK6和Survivin蛋白的表达量明显下调（P<0.05）。综上所述，PPⅦ通过抑制DLBCL细胞的增殖、促进细胞凋亡以及阻滞细胞于G0/G1期，发挥了抗淋巴瘤的作用。.