%0 Journal Article
%T Use of Whole Genome Sequencing for Mycobacterium tuberculosis Complex Antimicrobial Susceptibility Testing: From Sequence Data to Resistance Profiles.
%A Dreyer V
%A Sonnenkalb L
%A Diricks M
%A Utpatel C
%A Barilar I
%A Mohr V
%A Niemann S
%A Kohl TA
%A Merker M
%J Methods Mol Biol
%V 2833
%N 0
%D 2024
%M 38949712
暂无%R 10.1007/978-1-0716-3981-8_18
%X Whole genome sequencing of Mycobacterium tuberculosis complex (MTBC) isolates has been shown to provide accurate predictions for resistance and susceptibility for many first- and second-line anti-tuberculosis drugs. However, bioinformatic pipelines and mutation catalogs to predict antimicrobial resistances in MTBC isolates are often customized and detailed protocols are difficult to access. Here, we provide a step-by-step workflow for the processing and interpretation of short-read sequencing data and give an overview of available analysis pipelines.