%0 Journal Article
%T Temperatures above 37°C increase virulence of a convergent Klebsiella pneumoniae sequence type 307 strain.
%A Müller JU
%A Schwabe M
%A Swiatek LS
%A Heiden SE
%A Schlüter R
%A Sittner M
%A Bohnert JA
%A Becker K
%A Idelevich EA
%A Guenther S
%A Eger E
%A Schaufler K
%J Front Cell Infect Microbiol
%V 14
%N 0
%D 2024
%M 38947124
%F 6.073
%R 10.3389/fcimb.2024.1411286
%X <B>UNASSIGNED: </B>Convergence of Klebsiella pneumoniae (KP) pathotypes has been increasingly reported in recent years. These pathogens combine features of both multidrug-resistant and hypervirulent KP. However, clinically used indicators for hypervirulent KP identification, such as hypermucoviscosity, appear to be differentially expressed in convergent KP, potential outbreak clones are difficult to identify. We aimed to fill such knowledge gaps by investigating the temperature dependence of hypermucoviscosity and virulence in a convergent KP strain isolated during a clonal outbreak and belonging to the high-risk sequence type (ST)307.<BR><B>UNASSIGNED: </B>Hypermucoviscosity, biofilm formation, and mortality rates in Galleria mellonella larvae were examined at different temperatures (room temperature, 28°C, 37°C, 40°C and 42°C) and with various phenotypic experiments including electron microscopy. The underlying mechanisms of the phenotypic changes were explored via qPCR analysis to evaluate plasmid copy numbers, and transcriptomics.<BR><B>UNASSIGNED: </B>Our results show a temperature-dependent switch above 37°C towards a hypermucoviscous phenotype, consistent with increased biofilm formation and in vivo mortality, possibly reflecting a bacterial response to fever-like conditions. Furthermore, we observed an increase in plasmid copy number for a hybrid plasmid harboring carbapenemase and rmpA genes. However, transcriptomic analysis revealed no changes in rmpA expression at higher temperatures, suggesting alternative regulatory pathways.<BR><B>UNASSIGNED: </B>This study not only elucidates the impact of elevated temperatures on hypermucoviscosity and virulence in convergent KP but also sheds light on previously unrecognized aspects of its adaptive behavior, underscoring its resilience to changing environments.