%0 Journal Article
%T Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafish.
%A Qu ZL
%A Zhang YB
%J STAR Protoc
%V 5
%N 3
%D 2024 Jun 26
%M 38941183
暂无%R 10.1016/j.xpro.2024.103156
%X A gene-rescue experiment under a mutant background is essential to clarify gene function and the resulting biological potential in vivo. Here, we present a protocol for determining the change in interferon response by microinjecting plasmids into one-cell-stage zebrafish embryos. We describe steps for comparing the resistance potential to virus infection in wild-type and knockout zebrafish larvae following plasmid microinjection. We then detail how to link the enhanced interferon immunity to the improved resistance in knockout zebrafish larvae by gene-rescue experiments. For complete details on the use and execution of this protocol, please refer to Qu et al.1.