%0 Journal Article
%T Fungal Secretomics Through iTRAQ-Based Analysis.
%A Zheng L
%A Wang R
%A Fei Y
%A Pan Y
%A Zhou P
%A Shen R
%A Lan P
%J Methods Mol Biol
%V 2820
%N 0
%D 2024
%M 38941021
暂无%R 10.1007/978-1-0716-3910-8_13
%X Our understanding of how fungi respond and adapt to external environments can be increased by the comprehensive data sets of fungal-secreted proteins. Fungi produce a variety of secreted proteins, and environmental conditions can easily influence the fungal secretome. However, the low abundance of secreted proteins and their post-translational modifications make protein extraction more challenging. Hence, the enrichment of secreted proteins is a crucial procedure for secretome analysis. This chapter illustrates a protocol for iTRAQ-based quantitative secretome analysis describing the example of fungi exposed to different environmental conditions. The fungal-secreted proteins can be extracted by combining ultrafiltration and TCA-acetone precipitation. Subsequently, the secreted proteins can be identified and quantified by the iTRAQ-based quantitative proteomics approach.