%0 Journal Article
%T Immunosuppressive alkaloids from Narcissus tazetta subsp. Chinensis and the mechanism of (+)-narciclasine in vitro and in vivo.
%A Wang WL
%A Wu XY
%A Luo XY
%A Tang YQ
%A Cui J
%A Huang XY
%A Jiang YC
%A Liu Y
%A Li LM
%J Phytochemistry
%V 225
%N 0
%D 2024 Sep 25
%M 38936528
%F 4.004
%R 10.1016/j.phytochem.2024.114198
%X Three previously undescribed and sixteen known alkaloids were bioguidedly isolated from the bulbs of Narcissus tazetta subsp. chinensis (M.Roem.) Masamura & Yanagih. The structures were elucidated by spectroscopic data, including HRESIMS, NMR, and ECD. Eleven of the isolated alkaloids exhibited immunosuppressive activity on the proliferation of human T cells. (+)-Narciclasine (18) showed the most significantly suppressive activity with an IC50 value of 14 ± 5 nM. In vitro, (+)-narciclasine (18) blocked NF-κB signal transduction, but did not affect PI3K/AKT signal transduction. What was more, (+)-narciclasine significantly reduced ALT and AST levels and alleviated liver damage induced by ConA in AIH mouse model.